1. The purpose of Antibiotic Activity Test

The purpose of the test is to check that the raw ingredients or the manufactured products have the ability to inhibit the growth of bacteria that may cause inflammations on the skin such as Escherichia coli, Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, as well as determine the minimum concentration of the antibiotic compound.

2. Testing Method

Measurement of the Clear Zone
The clear zone is measured using paper disc method.
Take some of the each bacteria grown on the plate badge with the platinum loop and grow once again in 10ml of liquid badge for 18~24 hours.
Activate the bacteria and take 0.1ml of the bacteria to cultivate once again in 10ml liquid badge for 3~6 hours. Inoculate and spread 1×107 cells per plate with sterilized cotton applicator.
Place sterilized filter paper disc on solid plate badge and absorb the specimen (0.05ml / disc) according to concentration at 35℃ for 18~24 hours. Measure the diameter of the clear zone (mm) around the disc.